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The sequenced clones were analyzed using a standard nucleotide BLAST search of GenBank for homology with known bacterial 16S rDNA sequences.
In the present study, we analyzed bacterial 16S rDNA sequences using a standard nucleotide BLAST search of GenBank for homology with known bacterial 16S rDNA sequences.
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To minimize the problem generated by substitution saturation, nucleotide sequences can be translated into amino acid sequences; then they can be translated back into a nucleotide sequence using a standard genetic code, essentially getting rid of any synonymous substitutions.
Excess primers and nucleotides were removed using a standard SAP dephosphorylation protocol (USB, USA).
A section of the gel was cut out that corresponded to RNA of 16 30 nucleotides, as judged using a standard oligonucleotide marker.
Nucleotide binding was measured using a standard filter-binding assay.
For both nucleotide states, the average of the set using a standard deviation of 15 gave the best match, indicating that the angular disorder is ∼15°.
The synthetic nucleotide sequence, named DP, was incorporated into the expression vector pCMV6-XL5 using a standard DNA recombination procedure, resulting in the recombinant plasmid pCMV-DP.
He denied using a standard background.
using a standard sizing gradient.
Participants responded using a standard computer keyboard.
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