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Slides were photographed using a standard fluorescence microscope.
Activity of proteases was verified by using a standard fluorescence casein based assay (Molecular Probes).
Images were made using a standard fluorescence microscope (Carl Zeiss AG, Jena, Germany).
Fluorescence was detected using a standard fluorescence plate reader in the presence or absence of the specific caspase inhibitor.
Cells were stained with anti-cytokeratin phycoerythrin/DAPI/CD45-allophycocyanin (Dako, Carpinteria, CA, USA) and then manually counted using a standard fluorescence microscope (Olympus, Center Valley, PA, USA).
We also note that the RNA foci were not visible using a standard fluorescence microscope, but were readily visible with a confocal microscope, which may also explain differences with previous studies.
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Treated tumour cells were examined for degree of apoptosis and secondary necrosis using a standard fluorescence-activated cell sorting assay (Annexin V-FITC detection kit; Immunotech, Marseille, France), which detects binding of annexin V (A) and propidium iodide inclusion/exclusion.
Samples were analyzed under a fluorescence microscope using a standard fluorescent filter set at 520 ± 20 nm.
Boyden and his colleagues used the method to perform super-resolution imaging of a 10 million cubic micrometer chunk of tissue from the mouse hippocampus, stained with antibodies against three different neuronal proteins, using a standard confocal fluorescence microscope.
A straightforward method to achieve super-resolution consists of taking an image sequence of stochastically blinking emitters using a standard wide-field fluorescence microscope.
Two days after transfection, BDNF/mitochondria/TfR transport was analyzed using a standard wide-field fluorescence microscope equipped with a cooled charge-coupled device camera and controlled by MetaMorph (Molecular Devices, Sunnyvale, CA) according to Kwinter et al. [ 38].
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