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IgG antibodies against EBV viral capsid antigen (VCA) were detected using a standard Enzyme Linked Immunosorbent Assay kit.
Serum concentrations of interleukin 6 were measured using a standard enzyme linked immunosorbent assay (Sanquin, Amsterdam, the Netherlands, detection limit 4 pg/ml, coefficient of variation 5-105-10%
IFN-γ levels in frozen cell culture supernatants were evaluated after 5-day stimulation with the same antigens using a standard enzyme linked immunosorbent assay (ELISA R&D Systemss).
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Soluble SC5b-9 complement activity in plasma fractions was measured using a standard enzyme-linked immunosorbent assay (ELISA) that included specific anti-complement antibodies.
Purified recombinant proteins were assayed for PGM activity in the glycolytic direction (3-PG to 2-PG) using a standard enzyme-coupled assay as described previously [19].
Levels of DENV-2 envelope (E) protein-specific antibody in the serum of DENV-infected, uninfected engrafted and non-engrafted mice were determined using a standard Enzyme-linked immunosorbent assay (ELISA).
Ebola virus specific immunoglobulin (Ig) G was detected by using a standard enzyme-linked immunosorbent assay (ELISA) method as previously described (8 ).
All six specimens were initially tested for measles-specific immunoglobulin M (IgM) antibody using a standard enzyme-linked immunosorbent assay (ELISA).
H. pylori infection was screened serologically using a standard enzyme-linked immunosorbent assay and confirmed by histologic examination, biopsy urease test, urea breath test or bacterial culture.
Additionally, immunoglobulin-G antibodies to EBV-VCA (R-Biopharm AG, Darmstadt, Germany) were confirmed by serology testing using a standard enzyme-linked immunosorbent assay for both case and control subjects.
VEGF was measured in the urine and plasma using a standard enzyme-linked immunosorbent assay (ELISA) (R&D Systems) before each Suramin dose and was compared with that detectable after each Suramin dose.
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