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The glucosamine content was measured from 1.5 g (wet weight) for the glucose culture and from the content of the full culture for the wheat straw cultures and the dry weight was calculated back using a standard curve of the glucosamine content against the dry weight.
The absorption of the 15 dilutions was measured successively in the direction of ascending methylene blue content using a standard photometer U-20100, Hitachi, Ltd., Tokyo, Japan).
Quantification of L-phe was performed by comparison with external standards (0 250 mmol·L−1) and the lower limit of detection was 2.7 μM. Values were normalized for protein content using a standard Bradford assay.
These data were used to estimate the phenolic contents using a standard curve obtained from various concentration of Gallic acid.
Epulopiscium cells were manually selected from fixed intestinal contents, using a standard Gilson pipettor and a Nikon SMZ-U dissecting microscope.
The UV absorbance at 620 nm was recorded and the phenol content determined using a standard curve and function calculated using sterile phenol standards.
Cytoplasmic and nuclear protein extracts were prepared using a standard protocol [29] and protein content of each sample determined using the Micro Lowry Total Protein Kit (Sigma, according to the manufacturer's instructions).
The digested samples were used to measure the DNA content by using a standard curve (R = 0.999) prepared using calf thymus DNA standard and fluorescence dye (Hoechst 33258).
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