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The equipment was calibrated using a standard calibration ball (3.18551 cm) with errors of ±0.05%.
The drug concentration was calculated using a standard calibration curve.
Compounds were quantified using a standard calibration curve.
The glucose concentration was found using a standard calibration curve of the biosensor.
The amount of asiatic acid was determined using a standard calibration curves.
The absorbance values were converted to nitrate concentration using a standard calibration curve made with NaNO3 in the range 0 10 mM.
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Alternatively if we used a standard calibration procedure, we would have to use Zero Gas (air without any CO, SO2, NOX and O3) along with Span Gases (air with known concentration of specified gases) (Anderson Gerald&Hadden David1999) for calibration procedure.
These intrinsic parameters can easily be estimated using a standard camera calibration method (e.g., Bouguet [27]).
Quantification for all compounds was performed using a standard addition calibration with linear regression and isotopically-labelled internal standards between 0.25 and 1 μg L−1.
The luminescence, defined as the light emission recorded in the absence of excitation light, was quantitatively converted into luciferase protein using a standard luminescence calibration curve prepared by microinjection of eggs with known amounts of luciferase protein (Sigma) [ 15, 16].
The scanner was calibrated by measuring atomic steps on highly orientated pyrolytic graphite in the z-axis and using a standard 1-µm calibration grid (Agilent, USA) in the xy-plane.
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using a standard sizing
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