Sentence examples for using a standard calcium from inspiring English sources

To determine the responsiveness of MmTLR-4 and CaTLR-4 to LPS, HEK293T cells were seeded in poly-l-lysine-coated 96-well plates and transfected in triplicate using a standard calcium phosphate transfection protocol.

Transfections were done using a standard calcium phosphate precipitation technique.

EHEC O157∶H7 strain EDL933 was transformed with pKD46 using a standard calcium chloride method.

Cells were transfected using a standard calcium phosphate precipitation method using 0.2 µg DNA per 35 mm dish) for electrophysiological experiments, or Lipofectamine (Invitrogen) using 1.5 µg DNA per 60 mm dish for cell surface biotinylation experiments.

For standard ISGylation experiments in Hek239T cells, 3,5×105 cells were seeded the day before transfection in 6-well plates and transfected for 6 h using a standard calcium phosphate precipitation procedure.

Recombinant lentivirus and retrovirus vectors (10 µg) were packaged in 293T cells (3.3×106 cells per 10 cm dish) by co-transfection with either 2 µg of pCG-VsVG and 8 µg pCMVΔ8.2 [30], or with 2 µg pCG-VsVG and 8 µg pCG-GagPol [31], using a standard calcium phosphate based protocol.

Cells were transfected for 18 h using a standard calcium-phosphate protocol.

We have performed those experiments using ionomycin, a standard calcium ionophore, but we could not rescue the deficiency in cytokines as we predicted.

Serum Ca2+ concentrations were obtained using a standard curve of calcium carbonate and expressed as milligram per deciliter (mg/dl) of serum.

Using a standard curve of known calcium dilutions, the concentration of calcium for each cell line's complete medium was determined.

To ensure equivalent caloric intake in all animals, the Rapamycin group was pair-fed to the Control animals by providing the amount of food each day to Control that had been consumed the previous day by the Rapamycin treated rats using a standard rodent diet (23.4% protein, 0.6% calcium, 0.6% phosphate) (Purina Mills, Indianapolis, IN).