Sentence examples for using a standard alkaline from inspiring English sources

Plasmid DNA was isolated using a standard alkaline lysis protocol.

Briefly, BAC DNA was extracted using a standard alkaline lysis protocol developed by Genoscope (Evry, France).

Plasmid DNA from cultures was isolated using a standard alkaline lysis procedure (Sambrook et al. 1989).

Cosmid 7-2-1 was prepared using a standard alkaline lysis procedure.

Plasmid DNA was isolated from an overnight culture in liquid selective medium using a standard alkaline lysis procedure [ 72].

BAC DNA isolation was conducted using a standard alkaline lysis procedure on a QIAGEN BIO ROBOT 9600 (Qiagen, Valencia, CA, USA).

Plasmid DNA was isolated from BAC clones using a standard alkaline-lysis protocol and digested with 40 U of H indIII for 4.5 hr.

Fosmid DNA was recovered using a standard alkaline-lysis protocol, and sequenced using standard dye-termination methods and capillary electrophoresis according to ABI manufacturer's directions using a 3730xl Genome Analyzer.

Fosmid DNA was recovered using a standard alkaline-lysis protocol, and sequenced according to ABI manufacturer's directions, in an 8 μL reaction using 0.5 μL BDT version 3.1, 5 pmol of vector end-sequencing primers, and 100 ng DNA per reaction.

The assay was again developed without the addition of an anti-tag secondary antibody using a standard colorimetric alkaline phosphatase substrate, NBT/BCIP.

Immunohistochemistry of paraffin sections was carried out using a standard streptavidin biotinylated alkaline phosphatase (ABC-AP, DakoCytomation, Hamburg, Germany) method or tyramide signal amplification followed by ABC-AP (only for FoxP3).