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Room temperature was measured using a standard alcohol thermometer.
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Genomic DNA was extracted from peripheral blood lymphocytes using a standard chloroform isoamyl alcohol protocol.
DNA was extracted from the fat tissue samples using a standard phenol/chloroform/isoamyl alcohol technique.
From non-malignant paraffin-embedded tissues, DNA was extracted using a standard phenol/chloroform/isoamyl alcohol protocol [ 7].
From non-malignant cells in paraffin-embedded tissue, we extracted DNA using a standard phenol/chloroform/isoamyl alcohol protocol [ 29].
From nonmalignant cells in paraffin-embedded tissue, we extracted DNA using a standard phenol/chloroform/isoamyl alcohol protocol [ 42].
From non-malignant cells in paraffin-embedded tissue, we extracted DNA using a standard phenol/chloroform/isoamyl alcohol protocol (Isola et al, 1994).
DNA was extracted from 4 ml whole blood using the QIAamp DNA Blood Maxi Kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions and nonmalignant cells in paraffin-embedded tissue using a standard phenol/chloroform/isoamyl alcohol protocol.
We extracted genomic DNA from giraffe biopsy samples using a standard phenol chloroform/isoamyl alcohol extraction protocol.
Since 2007, numerous studies have used knockout mice to determine the effects of specific genes on alcohol consumption, using a standard two-bottle choice procedure in which the animals can freely choose between a water bottle and an alcohol (i.e., ethanol -containing bottlethanol -containing tabottle.
DNA was extracted using a standard phenol-chloroform extraction followed by alcohol precipitation [33].
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using a standard peptide
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