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SEAP is a secreted (extracellular) recombinant protein that can be assayed in culture medium using a standard activity assay.
In the former, uTWEAK was elevated in subjects with LN at diagnosis compared with those with SLE but no renal disease, and correlated with the degree of clinical disease activity as measured using a standard activity index.
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To assess the effect of freezing, aliquots of the enzyme solution were frozen at −80°C and analyzed over a period of 15 days using a standard PepP activity assay.
As it has been shown that the Ni- claMP complex acts as a superoxide mimic, verification of complex formation in the presence of EGF can be confirmed using a standard SOD activity assay.
Using a standard curve, arginase activity was reported as mUnits/mg of protein.
The duration of the drug treatment was 96 h after which the treatment efficacy was assessed using a standard assay measuring metabolic activity (Promega, Mannheim Germany) to quantify cell survival in vitro.
So, an appropriate nurse-patient ratio can be determined by using a standard for both indirect activities and direct nursing hours.
The WHEL Study assessed physical activity with nine questions from the Women's Health Initiative on usual physical activity (recreational walking and light, moderate, and vigorous physical activity) using a standard frequency-by-duration item format [ 27].
Optical density was read at 405 nm using an X-Mark microplate spectrophotometer (Bio-Rad, Mississauga, ON, Canada) and translated into ALP enzyme activity using a standard curve generated with p-nitrophenol (Sigma-Aldrich) ranging in concentration from 0 to 20 mM.
hCE1 mutants were successfully expressed, purified, and shown to exhibit enzyme activity using a standard esterase substrate para-nitrophenylbutyrate (pNPB) [11], although wildtype hCE1 was ∼30-fold faster toward pNPB than the V146H/L363E mutant (660 nmol/min/mg vs. 21 nmol/min/mg).
Protein concentration in the subcellular extracts was determined before the determination of sirtuin activity using a standard Bradford method.
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