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The samples were irradiated with monochromatic Al Kα X-rays (1486.6 eV) using a spot size of 220 μm.
Both lasers operated at 7 Hz repetition rate, 3 J/cm2 fluence, 7 µm/s velocity, and using a spot size of 120 µm.
The external quantum efficiency (EQE) was measured with an Oriel Quantum Efficiency Measurement kit (QE-PV-SI) using a spot size of approximately 2 mm2.
The spot detection algorithm was applied to three-dimensional reconstructions of samples to identify cells using a spot size of 3 to 4 μm.
In triplicate, colonic epithelial cells were laser capture microdissected using a PixCell II LCM machine (Arcturus) using a spot size of 15 μm, 70 mW of power and 295 volts.
X-ray diffraction measurements were taken with an AXS BRUKER D8 system in Bragg Brentano θ 2 θ geometry using Co (Kα) radiation using a spot size of 0.5 mm and rotating the sample around the surface normal to compensate for possible texture effects.
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Monochromatic aluminum K-alpha x-rays where used with a spot size of 100 μm, the flood gun was used to limit charging effects.
A ×20 dark field objective lens was used, giving a spot size of about 50 μmφ.
A ×20 long-working distance objective lens was used, giving a spot size of about 5 μmφ.
The cryogen-cooled 585 nm and 595 nm PDL was used with a spot size of 10 mm or 7 mm and a pulse duration of 450 μs or 1.5 ms at a fluence of 3.5 J/cm to treat surgical scars starting on the day of suture removal [ 12– 12].
The 585 nm PDL was used with a spot size of 10 mm with 10% overlap and a pulse duration of 0.5 ms at a fluence of 4.5 J/cm, in combination with treatment with the 1064 nm Nd YAG laser with a spot size of 10 mm and a pulse duration of 15 ms at a fluence of 60 J/cm, with 500 ms time delay between the delivery of the two wavelengths.
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