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The substrate specificity of the histone methyltransferase G9a was evaluated using a SPOT membrane encompassing residues 1 20 of H3 with numerous mutations and PTMs.
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Briefly, each protein spot on SYPRO Ruby stained gels was picked using a spot picker (Amersham Biosciences).
The determination of the epitope of the Amyloid-β specific antibodies was performed using a peptide spot membrane.
Use a spot treatment.
Use a spot stick.
Use a spot concealer to cover acne.
Peptides were synthesized on cellulose membranes using a MultiPep SPOT peptide arrayer (Intavis).
Membranes Synthesis Peptides for the first two arrays were synthesized directly on cellulose membranes (Intavis) using a MultiPep SPOT peptide arrayer (Intavis) and commercially available standard (Intavis) or modified (Bachem) L-amino acid precursors as previously described (Nady et al., 2008 ).
All fragments were spotted onto a 20 × 20-cm Nytran SuPerCharge nylon membrane (Schleicher and Schuell, Keene, NH, USA) using a BioGrid spotting robot (BioRobotics) in a 4 × 4 grid organization.
Spots were excised using an Ettan™ Spot Picker robot (GE Healthcare).
Spots were quantified using an automated spot counter (AID).
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