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Sterilized onion epidermis and detached rice leaves using a spot inoculation method can also be used to monitor penetration and invasion (Fig. 2K).
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Briefly, each protein spot on SYPRO Ruby stained gels was picked using a spot picker (Amersham Biosciences).
Use a spot stick.
Use a spot treatment.
Use a spot concealer to cover acne.
P. digitatum and P. italicum spores were inoculated (4.00 4.50 log cfu/orange) onto Washington navel oranges (Citrus sinensis L. Osbeck cv Washington navel) by using wound and spot inoculation methods and subjected to eight different UV-C doses in the range of 0.26 15.84 kJ/m2.
10 μl of the inoculum suspension was used for spot inoculation of the agar screening plates.
Pictures were taken using a RTcolor Spot camera (Diagnostic Instrument Inc) with Spot Advance v3.0 software.
The prepared plates were inoculated with C. aeruginascens using a four-point inoculation.
Similar experiments were performed using an intratracheal inoculation method.
Spots were quantified using an automated spot counter (AID).
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