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The voltage was set to 125 V. Sensitivity to the antifungal drugs FK506 and fluconazole was assessed using a spot growth dilution assay.
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Briefly, each protein spot on SYPRO Ruby stained gels was picked using a spot picker (Amersham Biosciences).
Still, CAPPIA slides can be printed with each containing up to 900 different preys as single spots when using a spot to spot distance of 1 mm.
Images were archived using a SPOT-2 image camera.
The oligonucleotides were subsequently spotted onto poly-Lysine coated glass slides at random positions using a spotting machine (GeneMachines, San Carlos, CA).
Use a spot treatment.
Use a spot stick.
Use a spot concealer to cover acne.
Use a spot of paint or a wooden stake.
Using the spot growth assay we demonstrate that 100 nM of rapamycin induced a noticeable inhibition of growth in cells harboring the empty vector but that cells having the 14-3-3 14-3-3 14-3-3ng plasmid grew normally (Supplementary Figure S2).
Using the spot growth assay, we show that yeast cells lacking Bmh1 or Bmh2 show very little growth when compared with wild-type cells in the presence of rapamycin.
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