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Fish were imaged on a Zeiss Axioplan 2 microscope using a Spot camera and Spot Advanced Imaging software (version 4.0.6).
Images were captured using a SPOT camera and SPOT software version 3.5.4 (Diagnostic Instruments, Inc., USA).
Images of the immunostaining were obtained using a SPOT camera mounted on a Zeiss Axioplan 2 microscope.
A time-series of images were taken using a SPOT camera on a Leica DM IRB inverted microscope with differential interference contrast microscopy and an ×20 0.4NA objective.
For DIG-labeled in situ hybridization, images were captured using a SPOT camera system (Diagnostic Instruments Inc., Sterling Heights, MI) attached to a Carl Zeiss Axioskop2 Plus light microscope.
Digital images were acquired using a Spot camera, and the accompanying image analysis software (Diagnostic Instruments, Inc., Sterling Heights, MI) attached to a Nikon Eclipse 50i microscope (Nikon, Tokyo, Japan) was used to process the images.
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Images of forming tubes were captured after 12 hours of incubation using a Spot-camera-equipped inverted microscope.
Images of individual spots were obtained by using a SPOT RT camera with SPOT-Advance software (Diagnostic Instruments, Serling Heights, MI).
Sections and whole mount ovules were examined using a Zeiss Axioplan microscope and digital images were collected using a Spot II camera.
Images were captured using a SPOT RT camera and a Nikon Eclipse 80i microscope.
Photomicrographs were captured by using a Spot CCD camera (Diagnostic Instruments, La Jolla, CA, USA).
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