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Garcia, A. J., Ducheyne, P. & Boettiger, D. Quantification of cell adhesion using a spinning disc device and application to surface-reactive materials.
The main aim of this work is to show the usefulness of a Computational Fluid Dynamics (CFD) model to interpret the results obtained from a reaction-precipitation process by using a spinning disc reactor (SDR).
Live imaging was carried out using a spinning disc coupled to an Olympus BX-41 microscope (Ropert Scientific, France) (20×, NA 0,5 or 40×, NA 0,75 objective, CoolSnapHQ2 camera) driven by Metamorph software (Universal Imaging).
The z-stack and 3D animation were done by using a spinning disc confocal microscope (Andor).
Immunostained brain sections were divided into rostral (hippocampus absent) and caudal slices (hippocampus present), examined and photographed using a spinning disc confocal fluorescent microscope (BX62 DSU; Olympus, Tokyo, Japan) equipped with Plan Fluor objectives, connected to a camera (Hamamatsu Orca).
Microscopic inspection of FITC and DAPI fluorescence was done using a spinning disc confocal microscope (Cell Observer® SD; Yokogawa CSU-X1) and using the program Zen 2011 (Carl Zeiss) for evaluation of images.
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We used a spinning disc confocal, which offers a higher acquisition rate and lower photobleaching than a point confocal.
The second system used a spinning disc confocal system (Visitech International, Sunderland, UK), collecting images at up to 30 Hz using a Hamamatsu C9100/13 EM charge-coupled device camera and Visitech software run on a Dell personal computer.
Time-lapse imaging was performed using a spinning-disc microscope (PerkinElmer Spinning Disk UltraVIEW ERS) with a 60× water (1.3 NA) objective or an inverted confocal microscope (Olympus FV1000) with a 60× silicon (1.35 NA) objective.
An avid reader of science magazines as a teenager, he became interested in the problem of television and was convinced that mechanical systems that used, for example, a spinning disc would be too slow to scan and assemble images many times a second.
The cracker, which consists of a hopper fitted with a flow rate control device, a cracking unit, winnowing unit and power system, operates on the principle of impact using centrifugal force from a spinning disc.
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