Microscopic inspection of FITC and DAPI fluorescence was done using a spinning disc confocal microscope (Cell Observer® SD; Yokogawa CSU-X1) and using the program Zen 2011 (Carl Zeiss) for evaluation of images.
To further dissect the differences in mitochondrial localization, we stained WT and six PXN mutant cells (P52L, A127T, P233L, T255I, D399N, and P487L) with MitoTracker Red and imaged the cells using a spinning disc confocal microscope at different z-planes.
In order to investigate whether AsiSI-induced DSBs may also exhibit clustering, we developed a DIvA cell line that expresses the 53BP1 repair protein fused to GFP, to follow DSB movement within the nuclear space by live cell imaging using a spinning disk confocal laser microscope.
The leading protrusion from PDAC cells within the junction between Met5A cells were imaged using a spinning disk confocal scan head (Yokogawa CSU-10) attached to an Nikon A1 inverted microscope.
Cells were imaged using a spinning disk confocal microscope (Zeiss, Thornwood, NY, USA), equipped with a CSU-X1A 5000 spinning disk unit (Yokogowa Electric Corporation, Tokyo, Japan), multilaser module with wavelengths of 458, 488, 514 and 561���nm, and an Axio Observer Z1 motorized inverted microscope equipped with a precision motorized XY stage (Carl Zeiss MicroImaging, Thornwood, NY, USA).
Live cell images were collected using a spinning disk confocal microscope system, Nikon-Andor (Nikon, NY), and MetaMorph software.
A solar venture says it has developed a "spin cell" technology using specialized lensing and a rotating conical shape that could generate five times more electricity from a given amount of land than conventional solar methods.
Use a spinning wheel and create bowstring.
They can use a spinning attack to counter that tactic.
