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Emerson performs experiments every day, from simple microscopy to acid tests, pH gauging, and using a spectrophotometer to check enzymatic reactions.
The 1 h culture was diluted with saline using a spectrophotometer to achieve a cell density of 107 colony forming units (CFU) ml−1.
After 4 h, the suspensions were centrifuged, and the supernatants were analyzed using a spectrophotometer to determine the concentration of glucose through DNS method to obtain the amount of consumed glucose [16].
Total RNA concentration was determined using a spectrophotometer to measure absorptions at 260 nm and 280 nm wavelengths.
Absorbance at 260 and 280 nm was measured using a spectrophotometer to determine the concentration and purity of RNA.
The optical density of samples was measured at 405 nm using a spectrophotometer to determine the degree of contact activation on the different surfaces.
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We used a spectrophotometer to obtain reflectance spectra of the ground color of the eggs.
Next, a 100 μL aliquot was taken from each well and placed into 96-well plates to be read using a spectrophotometer at 595 nm wavelength, according to Ocarino et al. [ 10].
A 100 μL aliquot was then taken from each well and placed into 96-well plates to be read using a spectrophotometer at 540 nm wavelength, according to Boeloni et al. [ 20].
The density of the suspension was adjusted using a spectrophotometer at wavelength of 520 nm to a transmittance of 70 to 72%.
The absorbance was measured at 240 nm using a spectrophotometer adjusted to 25°C.
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