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The pNA light emission was quantified using a spectrophotometer plate reader at 405 nm.
Absorbance was measured at 570 nm using a spectrophotometer plate reader (Fluostar Optima).
The wells were then treated with ortho-phenylenediamine (OPD) substrate, and the absorbance at 492 nm was obtained using a spectrophotometer plate reader (Molecular Devices, Sunnyvale, CA).
The absorbance of the samples was measured using a spectrophotometer plate reader (Multilabel Counter, 1420 Victor, Perkin Elmer) at 570 nm and 600 nm.
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The plates were then incubated at room temperature for one hour in 100 μL of streptavidin-HRP enzyme conjugate (1 : 1000), and the plates were read immediately using a spectrophotometer microwell plate reader at 450 nm.
After 3 hours, the absorbance of dye eluted from viable cells was measured at 540 nm using a spectrophotometer Elisa plate reader (Molecular Devices EMax).
The optical density of the aliquots was measured at 570 nm OD570 nm using a spectrophotometer (VICTOR™X3 Multilabel Plate Reader; PerkinElmer Inc, Waltham, MA).
All liquid fractions were probed for GAGs by absorbance analysis using a spectrophotometer (FLUOstar Optima plate reader (BMG Labtech)) with 485 nm bandpass filter for f-HA and by dimethylmethylene blue dye method (DMMB) for ChS.
Absorbance at 355/445 nm was measured in a black 96-well plate using a spectrophotometer.
We measured the luminescence and fluorescence of cells cultured in a 96-well plate using a spectrophotometer and then calculated the caspase-3/7 activity per cell.
Glycosaminglycan content was determined by the di-methyl-methylene blue sulfated glycosaminoglycan assay [ 48] using a spectrophotometer (Synergy HT– KC4 Spectrophotometric Plate Reader and FT4software, BioTec, Winooski, VT).
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