The PCR amplifications were performed using a thermal cycler (Thermal Cycler; Bio-Rad).
Each version uses a specific gin.
Use a specific item to defeat it.
qPCR was performed using a MyIQ thermal cycler, IQ SYBRGreen SuperMix (BioRad, Veenendaal, the Netherlands) and dog-specific primers (Eurogentec, Maastricht, the Netherlands) (see Additional file 1).
Quantitative RT-PCR was performed using a TaKaRa Thermal Cycler Dice Real Time System (TaKaRa Bio, Ohtsu, Japan) and gene-specific primer sets.
MHC II specific anti-sense primers (Sigma) were used for reverse transcription reactions (RT) and PCR was performed using a Mastercycler thermal cycler (Eppendorf).
Amplification of the 18S rDNA gene was carried out by PCR using a thermal cycler (MJ Research, Inc., PTC-100 Programmable Thermal Controller) and eukaryotic specific primers (Medlin et al. 1988; Turbeville et al. 1992; Nygren & Sundberg 2003).
The thermal stability of the collagen fragments was monitored using a CD thermal denaturation experiment.
Thermal hyperalgesia was measured using a paw thermal stimulator (IITC Life Sciences, Woodland Hills, CA).
A PCR was performed using a Veriti thermal cycler (Life Technologies).
