Sentence examples for using a specific substrate from inspiring English sources

Moreover, this inhibition can be overcome by using a specific substrate concentration at which V max remains unaffected [35].

Midgut samples were tested for trypsin-like activity in solution assays using a specific substrate.

The enzyme activity of caspase-3 stimulated by osajin was measured using a specific substrate, DEVD-pNA.

Caspase-3 expression was determined by western blots and activity was determined spectrophotometrically using a specific substrate (DEVD-AMC).

Caspase-3 activity was spectrofluorometrically determined in cytosolic protein extracts using a specific substrate (Ac-DEVD-AMC) for this caspase, according to the supplier's instructions (BD Biosciences).

The filters were analysed for the content of the enzyme N-acetylhexosaminidase (NAHA) as a marker of fungal cell biomass, using a specific substrate and a fluorescent technique and expressed as NAHA units (U /m.

Activity was assayed in extracts from control and irradiated samples using a specific colorimetric substrate as described [26].

Next, to understand if Pam3CSK4 alone or its combination with Velcade influenced the enzymatic activity of caspase-3, we assayed its activity using a specific peptide substrate.

However, like others (Stingele et al., 2014) we could not detect such activity in Wss1 complexes using a specific Ub-SUMO substrate.

The kinetoplast DNA (kDNA) was used as a specific substrate for TOP2 enzymes.

The assay was performed by using a specific fluorescent caspase-3 substrate (DEVD-AFC) that on hydrolysis produces a green fluorescent product.