Sentence examples for using a specific sirna from inspiring English sources

To confirm this role, we knocked down the endogenous Sp1 using a specific siRNA.

UCP2 gene expression was knocked down using a specific siRNA transfection approach (Fig. 5A).

This was investigated by examining the effect of endogenous VEGF knockdown using a specific siRNA.

We inhibited B7-H1 using a specific siRNA before treating MDA-MB-231 cells with doxorubicin.

To verify the result, the same experiments were performed using a specific siRNA for the knockdown of NOTCH1.

B7-H1 expression was inhibited in MDA-MB-231 cells using a specific siRNA (CD274 siRNA ID = s26547 and siRNA ID = s26548, Ambion, Austin, TX, USA).

Thus, we knocked-down the expression of these molecules in Panc-1-EGFP and Panc-1-DsRed cells using a specific siRNAs (Fig 5) and we assessed HoCC after 48 h, with or without concomitant Nupr1-depletion and/or TGFβ treatment.

To determine the effect of CXCR4 silencing on the invasiveness and migration potential of MDA-MB-231 cells, we used a specific siRNA directed against CXCR4 mRNA.

This was confirmed using a specific LIF siRNA: while a scrambled siRNA showed no effect, blocking LIF expression significantly inhibit the TGFβ effect on melanoma cell migration by about 60%.

We then assessed if p21 had a role in this process using a specific p21 siRNA.

Furthermore, we found this effect to take place at the transcriptional level, as LIF gene expression knockdown using a specific LIF siRNA completely blocked TGFβ-induced p21 gene promoter activity.