Sentence examples for using a specific polymerase from inspiring English sources

Using a specific polymerase chain reaction (PCR) assay targeting a Tat domain protein in comparison with a bacterial culture method, we examined the prevalence of B. pseudomallei in soil samples from an oceanarium in Hong Kong where captive marine mammals and birds have contracted melioidosis.

Clinical isolates were screened using a specific 16S rRNA gene polymerase chain reaction (PCR) assay, and sequenced to confirm taxonomic identities.

The third step is the DNA amplification, called SPIA™ amplification using a specific DNA/RNA chimeric primer, DNA polymerase and RNase H in a homogeneous isothermal assay that provides highly efficient amplification of DNA sequences.

Loop-mediated isothermal amplification (LAMP) is a new method developed in year 2000 by Notomi et al. Basically, this method of DNA amplification uses a specific DNA polymerase enzyme and a set of four specific primers that distinguish six different regions on the sequence of the target DNA.

Acetylation genotype was determined on genomic DNA by using an allele-specific polymerase chain reaction-restriction fragment length polymorphism (PCR RFLP) assay.

HP genotyping was performed using an allele-specific polymerase chain reaction (PCR)–based method distinguishing the major allelic variants (Hp1 and Hp2 ) of the α-chain [ 12].

Based on the sequence data, we developed a kdr mutation diagnostic method using allele specific polymerase chain reaction (AS-PCR).

It has been shown, using highly specific polymerase chain reaction (PCR -based genotyPCR -basedds, that in TB patients in a higenotypingce setting, the samethodsum sample conthats dinferenTBM. tuberculosis strains [23].

Subtelomeric methylation level was primarily determined in triplicate using methylation specific polymerase chain reaction (methylation specific PCR (MSP)) and expressed as the methylation ratio.

DNAs were isolated from the fine needle aspiration cytology specimens and the methylation status of TSHr promotor region was investigated by using methylation specific polymerase chain reaction.

They used an allele-specific polymerase chain reaction assay as described by Myakishev et al. (21) (rs6313, rs3924999) or InvaderPlus reactions from Third Wave Technologies (Madison, Wisconsin) (rs4680, rs6311).