Sentence examples for using a specific pcr from inspiring English sources

The environmental isolates that were identified phenotypically as Mycobacterium were further verified to the genus level using a specific PCR protocol based on a 228-bp fragment of the 65-kDa heat shock protein (hsp65).

The strains were examined for the presence of class 1 integrons by using a specific PCR assay (9).

OvHV-2 DNA was detected by using a specific PCR (4 ) in 5 of 7 paraffinized sections of the brainstem (5 ).

Plasma samples were prepared as described previously (10 ), and extracted nucleic acids were tested for GemyC1c DNA by using a specific PCR that included negative, positive, and extraction controls (Technical Appendix).

Each exon of the kinase domain, plus a region containing the proteolytic cleavage site in exon 1, was examined for mutation using a specific PCR primer pair as described previously (Angeloni et al, 2000).

Subsequent clinical laboratory testing of the brain biopsy sample at the US Centers for Disease Control and Prevention CDCC), using a specific PCR targeting the RNAse P gene [ 42], confirmed the diagnosis of Balamuthia mandrillaris encephalitis.

The Ala592Thr variant was detected using a specific PCR-restriction fragment length polymorphism test.

We used a specific PCR method targeting the bacterial 16S ribosomal DNA (rDNA) region for this purpose.

All cell lines were routinely tested for contamination by Mycoplasma spp. using a specific multiplex PCR (PCR Mycoplasma Detection Set, Clontech Laboratories Inc., Mountain View, CA, USA).

Direct detection of M. lentiflavum in sputum samples was conducted using a specific real-time PCR assay.

Rhinovirus/enterovirus negative specimens and samples without sequencing results were additionally analyzed by using a specific EV-D68 PCR (12 ).