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The efficiency of the p21 siRNA was demonstrated by Western blot, using a specific p21 monoclonal antibody.
We then assessed if p21 had a role in this process using a specific p21 siRNA.
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Importantly, immnocytochemical analysis using a specific XRCC3 antibody confirmed the over-expression of the protein in the xenograft tumour tissues.
To confirm this, Tat and morphine induced toxicity to human neuroblastoma cells was analyzed after inhibiting the p38 pathway by using a specific p38 inhibitor, SB203580.
The protein lysate was immunoprecipitated using a specific Ago2 mouse monoclonal antibody (Abcam) and bound by A/G agarose beads (Santa Cruz Biotechnology).
Clinical isolates were screened using a specific 16S rRNA gene polymerase chain reaction (PCR) assay, and sequenced to confirm taxonomic identities.
The findings from this study specifically raised the possibility of using a specific p38γ activator to promote skeletal muscle metabolic function.
HSV-2 serology was performed using a specific IgG2 ELISA (Kalon Biologicals) with high sensitivity and specificity in African serum samples [ 25].
Downstream signaling via ERK1/2 mitogen activated protein kinase (MAPK) was examined using a specific MEK1/2 inhibitor.
The draft manual instructs NSA operators to track their use of one malware program using a specific 16-character string, "ace02468bdf13579".
An original experimental setup was designed to reproduce the underground conditions using a specific CO2 regulation device.
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