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CXCR4 was then blocked using a specific monoclonal blocking antibody.
The stability and proper folding of all the tested constructs expressed was confirmed by western blot, using a specific monoclonal antibody against the HA tag (Figure 2E).
We examined the autophagy conjugation system by detecting Atg12-Atg5 using a specific monoclonal antibody raised against a full-length recombinant Atg12.
We would like to point out that we detect AtTPK3, shown to exhibit high transcript level [30], in thylakoids obtained from genetically non-manipulated Arabidopsis plants, by using a specific monoclonal antibody.
The evaluation of the expression of sLex using a specific monoclonal antibody showed no detection of this carbohydrate antigen on the E-cadherin immunoprecipitate from canine mammary carcinoma cell line, CMT-U27 (Figure 3).
Expression of nm23-Hl gene product was determined using a specific monoclonal antibody.
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We used a specific monoclonal antibody directed against Mcm2 and a previously validated tissue microarray (TMA) with tissue samples of a large number of DLBCL [ 37].
All lung samples were stained for standard histopathological analysis and for immunohistochemical methods using a specific mouse monoclonal antibody.
The efficiency of the p21 siRNA was demonstrated by Western blot, using a specific p21 monoclonal antibody.
As a further control, the localization of TPK1 in Arabidopsis cells was assayed by using a specific anti-TPK1 monoclonal antibody.
Purity of isolated monocytes was assessed by flow cytometry (FACScan™, Becton Dickinson) analysing expression of the monocyte marker CD14 using a specific phycoerythrin-conjugated monoclonal antibody (BD Bioscience, Heidelberg, Germany).
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