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Cytokines were measured by using a specific immunoassay.
Serum SP-D levels were measured using a specific immunoassay.
The concentrations of VEGF (VEGF-A165) were determined in the conditioned media of cell culture using a specific immunoassay kit (R&D Systems) according to the manufacturer's instructions.
Faecal amylase was assessed using a specific immunoassay to identify human pancreatic amylase and showed a similar trend to FP with increased values which correlated with FP activity.
The supernatant was evaporated and reconstituted with assay buffer, and then LTC4 was purified through a Sep-Pak column (Waters Associates, Milford, MA, USA) and measured using a specific immunoassay (Cayman) according to the manufacturer's instructions.
Methods: We measured serum SP-D levels from patients with stable COPD (n = 14), patients experiencing acute exacerbations (n = 13) and in control subjects (n = 54) using a specific immunoassay and compared the levels using analysis of variance.
Similar(53)
The concentration of serum CD26 were analyzed using a specific immunoassays (Human CD26/DPP4 ELISA Kit, Boster biological technology, Pleasanton, CA) ELISAs were performed according to the manufacturer's instructions: mean values of duplicated measurements were calculated and a sigmoid-shaped standard curve was determined by simultaneously analyzing a dilution series of standard samples.
Furthermore, in this study, we used a specific immunoassay that detects only IgG-class anti-PF4/heparin antibodies to determine the frequency of these antibodies and their contributions to the occurrence of VTEs.
Plasma total cortisol was measured using a specific radio immunoassay (as previously described in Désautés et al [ 7]).
8-isoprostane concentrations were measured in duplicate using a specific enzyme immunoassay kit (Cayman Chemicals, Ann Arbor, MI, USA).
Therefore, to assess the importance of oxidative stress in the progression to TP patients, we measured, using a specific validated immunoassay in scraping samples, concentrations of 8-iso-PGF2α in a series of consecutive patients with TP.
More suggestions(15)
using a specific set
using a specific key
using a specific pipette
using a competitive immunoassay
using a specific database
using a specific sequence
using a colorimetric immunoassay
using a specific product
using a specific developer
using a liposome immunoassay
using a specific mask
using a specific conversion
using a specific move
using a specific antibody
using a direct immunoassay
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