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Culture media were assayed directly for sRANKL using a specific ELISA kit (Peprotech EC – human sRANKL ELISA development kit), according to the manufacturers directions.
Nitrotyrosine and ADMA were determined using a specific ELISA kit (Chemicon).
CRP was assayed in serum by using a specific ELISA method, as recommended by the provider (ALPCO).
PVL production by the LAC strain was checked by using a specific ELISA method [21] in the supernatants of inoculum samples.
The growth of pancreatic tissues was determined in the SCID recipient mice by monitoring blood levels of pig insulin using a specific ELISA, previously shown to correlate with implant size[8].
PSP was determined using a specific ELISA.
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Using a specific ELISA-test we studied patients with small cell lung cancer (n=63), and patients with non-small cell lung cancer (n=22) in comparison to healthy control subjects (n=41).
We thus used a specific ELISA developed to quantify the protein levels expressed by adherent cells isolated from the two tissues.
Antibodies against L. intracellularis were detected using a specific blocking ELISA.
IL-17A levels were determined using a specific mouse Elisa kit (Cat 88-7371; eBioscience, San Diego, CA, USA).
TNFα was then quantified in the media using a specific TNFα ELISA kit.
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