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The efflux of excess cysteine using a specific cysteine exporter is another mode of regulation.
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Inhibition of StpC activity during bacterial growth using a specific irreversible inhibitor of cysteine peptidases (E-64) had no effect on the level of BacCH91, suggesting an StpC-independent maturation mechanism.
Maturation and Clearance of Autophagosomes in Neurons Depends on a Specific Cysteine Protease Isoform, ATG-4.2.
Each version uses a specific gin.
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As most endolysins exhibit a similar modular structure, we believe that our work emphasizes the inability to improve the in vivo half-life of this class of enzybiotics using a cysteine-specific PEGylation strategy.
Activity of SpeB was determined by active site titration using the specific cysteine proteinase inhibitor L-trans-epoxysuccinylleucylamido(4-guanidino)butane 4-guanidino butaneviously described [54-guanidino butane
To minimize the proteolytic activity SpeB might have on cells, we terminated the reactions using the specific cysteine proteinase inhibitor E-64 [43].
To see if a similar protease processing of virions occurs within Raji cured tumours, which results in their susceptibility to killing by reovirions in vivo, we used the specific cysteine protease inhibitor E64, which has been shown to block the conversion of reovirions to ISVPs (Baer and Dermody, 1997; Chandran and Nibert, 1998).
Similar fragmentation of a cysteine-HNE Michael adduct was reported earlier using a deuterated cysteine (40).
The final cysteine concentration was adjusted to 2% using a 40% cysteine solution.
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