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Contigs and scaffolds were concatenated at random using a spacer sequence of 150 N's.
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Till date, either a portion of the sequence from gene III from the phage M13mp19 [10] or a portion of the helical region of tolA [11] or the extended region of tonB [12] from E. coli has been used as a spacer sequence.
The yellow gene was used as a spacer sequence.
The up- or down-stream sequences of gene 1 were concatenated to the up- or down-stream sequences of gene 2 using a spacer of 20 'N' nucleotides.
We determined a set of E. coli promoters that were potential candidates to operate in tandem (sometimes using a suitable spacer sequence to isolate them).
Use a spacer to keep that distance even.
To search pseudogenes, TBlastN searches were conducted against the E. coli protein data set using spacer sequence between estimated open reading frames (ORFs) in the symbiont genomes as query.
With increasing spacer length, the immunogenicity decreased when using optimal spacer sequences.
All therapeutic epitopes could be recovered using the in silico designed spacers with a smaller or equal number of neo-epitopes compared to the best experimentally used spacer sequence.
To determine the physical position of Nor-B2 in detail, we developed one DNA marker using the intergenic spacer sequences (IGSs) between the 18S and 25S rRNA genes [ 44].
For simplicity, most SBV designs have so far used fixed spacer sequences.
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