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The solutions were sonicated for 5 min using a sonicator probe (220 240 V, 10.3 A Philip Harris Scientific, Lichfield, UK) to break up aggregation before dilution to different surface area doses, calculated from NP surface area.
The emulsion was then sonicated at an amplitude of 50% level for 5 min using a sonicator probe (QSonica Q700).
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Cell suspensions were sonicated at 150 kHz, using a sonicator with a 13 mm probe.
To prepare ESC extracts, cells were washed twice in PBS supplemented with 0.1% protease inhibitor cocktail (Promega, Southampton, UK), sedimented at 1200 rpm then sonicated on ice using a sonicator fitted with a 3mm diameter probe until all cell and nuclei were lysed.
For the sonication technique, liposomes are prepared using a sonicator to mix the lipid suspension.
For the test fluids of this article, the particles and up to 1% sodium dodecyl sulfate (a surfactant) were dispersed into the base fluid using a sonicator (a UP200 from Hielscher Ultrasonics GmbH, Teltow, Germany) for 15 to 30 min. From our experience, probe-type sonicators break particle agglomerates faster and much more thoroughly than bath-type sonicators.
27 hours post infection, HeLa cells were harvested and lysed using a sonicator (Fisher).
Cell membranes were disrupted using a sonicator with a micro-tip (Soniprep 150, MSE London UK).
Dry powder (10 g) was extracted three times with methanol (100 ml) using a sonicator.
The biomass was pretreated using a sonicator to break down the cell walls.
Briefly, cells were disrupted using a sonicator 3000 (Misonix, Inc., NY).
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