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Upon thawing, cells were lysed using a sonication probe.
The cell suspension was lysed using a sonication probe (TT13/FZ, Bandelin Sonopuls HD3200) at 30% amplitude for 5 min, pulsed on for 10 s and pulsed off for 10 s.
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Infected cells were pooled and semi-purified using a sonication and centrifugation method prior to passage.
The strains were isolated using a sonication protocol previously described (Esteban et al. 2008).
In the present study, we washed hair with hot water and shampoo in a covered vessel using a sonication bath.
The suspensions were sonicated with a sonication probe (Sonics & Materials) at 32 W for 15 s before adding to the cells.
The double emulsion was then deal with sonication using a probe sonicator (Sonics & Materials, Newtown, CT, USA) for 5 min, followed by moderately stirred with a magnetic mixer for 10 h to solidify the nano-droplets.
All filtered bee samples were lysed using an ultra-sonication probe at settings of 20 seconds pulse-ON, 5 seconds pulse-OFF, and 25 percent amplitude for 5 minutes duration.
The cultures of three C. pecorum porcine isolates L1, L17 and L71 were propagated in chicken embryos and purified, as previously described [ 22], while the cultures of 14 koala isolates were propagated in Hep-2 and/or McCoy cells and semi-purified using a probe sonication and density gradient centrifugation, as previously described [ 1].
For example, Mili and Steitz [ 30] used a sonication method optimized for small nuclear ribonucleoproteins, whereas mRNA RIP-CHIP uses mild polysome lysis buffer and no sonication to avoid the shearing of large RNAs and their reassortment [ 29].
To measure the size, a suspension of NPs (0.5 mg/ml) in water was prepared by sonication using a microtip probe sonicator as above for 1 min.
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