Sentence examples for using a smart cycler from inspiring English sources
Amplification was performed with a One-Step SYBR RT-PCR Kit (Takara) according to the manufacturer's protocols using a Smart Cycler II System (Cepheid, Sunnyvale, CA).
Real-time PCR was performed using a Smart Cycler II (Cephid, Inc., Sunnyvale, CA).
Quantitative RT-PCR (qRT-PCR) was performed on each RNA sample using a Smart Cycler (Cepheid, Sunnyvale, CA) to detect any DNA contamination.
Real-time PCR reaction was performed with a Takara Ex Taq R-PCR kit, version 1.0 (Takara, Otsu, Japan), using a smart cycler system (Cepheid, Sunnyvale, CA, USA).
Real-time PCR was performed with a Takara Ex Taq R-PCR kit, Version 1.0 (Takara, Otsu, Japan), using a smart cycler system (Cepheid, Sunnyvale, CA, USA).
Real-time reverse transcription PCRs will be run using a Cepheid Smart Cycler II or similar instrument.
A laboratory diagnosis of influenza infection was determined by real-time reverse transcription PCR (RT-PCR), by using the Centers for Disease Control and Prevention's protocol, on a Smart Cycler II (Cepheid, Sunnyvale, CA, USA) (7 ).
After cDNA synthesis using Oligo dT 15 Primer (Promega protocol; Promega, Madison, WI, USA), 5 μL cDNA (1 5 or no dilution for Ppara in cell experiments) was used for quantitative polymerase chain reaction (qPCR) using SYBR green (Quanta Biosciences, Gaithersburg, MD, USA) in a Smart Cycler rapid thermal cycler (Cepheid, Sunnyvale, CA, USA).
Quantitative real-time RT-PCR was performed using specific primers for mouse Tem1 and Gapdh and a Smart Cycler (Cepheid, Sunnyvale, CA).
The real-time PCR was carried out with a Smart Cycler II (Cepheid, Sunnyvale, CA) using the following conditions: one cycle of 95°C for 10 min and 45 cycles of 95°C for 15 s, 55°C for 30 s, and 60°C for 30 s.
RT-QPCR was performed on a Smart Cycler (Cepheid, Sunnyvale, CA, USA) using cDNA synthesized from the cancer specimens and TaqMan probes (TaqMan Gene expression Assays, Life Technologies) according to the following protocol: 600 s at 95°C, followed by thermal cycles of 15 s at 95°C and 60 s at 60°C for the extension.
