Sentence examples for using a smart cdna from inspiring English sources

The cDNA clone of eel Cu/Zn SOD (eCu/Zn SOD) was obtained by using a SMART cDNA library construction kit (Clontech).

PCR-based cDNA libraries were constructed using a SMART cDNA library kit (BD-Clontech) as previously described [ 45].

The comparison here is between EST frequencies in libraries constructed by two different methods, the other libraries were constructed using a SMART cDNA kit.

Approximately 1 μg total RNA was converted into cDNA using a SMART cDNA synthesis kit (Clontech, Palo Alto, CA, USA), optimizing the conditions to obtain a large quantity of clean cDNA in a small volume.

The digested RNA was converted to cDNA using a SMART cDNA synthesis kit (Clontech, CA, USA) and then amplified by applying the Advantage II polymerase (Clontech, USA) to increase the total quantity of the sample for sequencing.

Briefly, first strand cDNA synthesis was carried out using a SMART cDNA Library Construction kit (Clonetech) according to the manufacturer's recommended protocol except for the use of a modified CDS-3 M adapter (5' – AAG CAG TGG TAT CAA CGC AGA GTG GCC GAG GCG GCC TTT GTT TTT TTT TCT TTT TTT TTT VN – 3').

DNA cloning of the CAHL gene was carried out using a SMART-cDNA isolation kit following the manufacturer's instructions (Clontech Laboratories, CA, USA).

The full-length sequence of PsANR was recovered by rapid amplification of cDNA ends (RACE) using a SMART RACE cDNA Amplification kit (Clontech).

Based on the assumption that the reproductive organ-specific transcripts may be relatively rare in the extracted mRNA population, the male cDNA was amplified using a SMART PCR cDNA Synthesis Kit (Clontech).

In brief, the RNAs from the same tissue of the six C. semilaevis individuals were pooled before the first-strand cDNA was synthesized using a SMART PCR cDNA synthesis kit (Clontech, USA).

Second, to amplify the 3' and 5' ends of the cDNA, sense and antisense gene specific primers (GSP) were designed based on the sequences obtained from the PCR products described above and then used for the 3' and 5' Rapid Amplification of cDNA Ends (RACE) using a SMART RACE cDNA Amplification Kit (Clontech).