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Knockdown of claudin-7 in SCC cell lines using a small interfering RNA approach led to decreased E-cadherin expression, increased cell growth, and enhanced invasion into a three-dimensional matrix.
Silencing of NUB1 was performed using a small interfering RNA.
Fibulin-5 was knocked down using a small interfering RNA (siRNA) duplexes (ON-TARGETplus siRNA J-017621-05 and -06; GE Dharmacon, Chicago, IL, USA).
Knockdown of Orai1 using a small interfering RNA (siRNA) approach reduced CRAC channel activity by ∼70% [ 29] and reduced Orai1 expression by 62% ± 7%.
Knockdown of endogenous PA28γ using a small interfering RNA (siRNA) pool (Dharmacon) increased the levels of endogenous p53 and p21Waf1 in LNCaP, HCT116, A549, MCF-7, and MCF10A (a normal breast epithelial line) cells, but not PC3 cells.
To further confirm the antiapoptotic activity of HO-1, we performed a separate experiment by using a small interfering RNA (siRNA) to suppress specifically the expression of HO-1.
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Next, we used a small interfering RNA (siRNA -induced knockdown to further analyze the participation of CLTC/AP2A1/AP2B1 in hTAC endocytosiRNA -induced
To further demonstrate that EWS/WT1 is directly regulating the ENT4 expression, we used a small interfering RNA (siRNA) approach to deplete the level of EWS/WT1 in JN-DSRCT-1 cells.
To confirm that both responses depended on Orai1, we first used a small interfering RNA (siRNA) knockdown approach to reduce expression of channel protein.
Next, we used a small interfering RNA (siRNA), which was designed according to a previous report in which PML-RARα was effectively knocked down by specifically targeting its breakpoint region [ 20].
To test this, we used a small interfering RNA (siRNA) targeted to human Eps8 that resulted in 76 ± 2.1% (mean ± SE) depletion of the protein in A375 cells after 24 hr (Eps8-KD, Figure 2A).
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using a small example
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using a single interfering
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