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Exact(32)
Leaf samples were cut using a sliding microtome, stained with Safranin-O and counterstained with Fast Green.
Subsequently, each tissue sample was cut into sections of 2 µm thickness using a sliding microtome (SM 2000 R; Leica Biosystems; Germany).
Sections of 40 μm were cut using a sliding microtome (Retoratome REM-700, Yamato Kohki Industrial Co., Ltd., Saitama, Japan) with a refrigeration unit (Electro Freeze MC-802A, Yamato Kohki Industrial Co., Ltd).
Frozen sections (50 µm) were cut using a sliding microtome.
40-µm sagittal sections were cut on dry ice using a sliding microtome.
Frozen sections (30 µm thick) were cut using a sliding microtome and collected in PB with azide.
Similar(28)
After the brains were removed and postfixed at 4°C overnight in fixative, they were soaked overnight in 0.1 M PB containing 25% sucrose for cryopreservation and subsequently sectioned in the horizontal plane at 30 µm, using a sliding knife freezing microtome (Microm, Germany).
Skin and dorsal root ganglia were post-fixed in 4% paraformaldehyde, cryoprotected in 25% sucrose, embedded in gelatin, sectioned on a sliding microtome and labeled using target-specific antibodies followed by a fluorescently tagged secondary.
The paraffin sections were cut on a sliding microtome at 4 μm per section.
Coronal sections of 40 μm were prepared with a sliding microtome.
Sectioning was performed on a sliding microtome.
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