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Images were captured under uniform light conditions using a slide scanner (Myer Instruments, Houston,TX).
Images were acquired by using a slide scanner and corresponding software (Mirax Midi; Zeiss, Göttingen, Germany).
Then the slices were scanned using a slide scanner at 4× magnification.
Stained sections were digitalized using a slide scanner (Nanozoomer 2.0-HT, Hamamatsu, Japan), and measurements of the cerebellar vermis and hemisphere thickness relative to whole thickness were performed using the NDPview software of the digital scanner.
Each slide was scanned using a slide scanner (3DHistech Ltd ,Budapest, Hungary)and viewed using the corresponding software on a computer.The degree of bone necrosis was assessed by counting the percentage of empty lacunae within a single strip of bone in the middle of each section.
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All slides were digitalised using a Mirax slide scanner and analysed using Panoramic Viewer (version 1.15.50, 3DHistec, Budapest, Hungary).
Overview pictures of histological slides were taken using a digital slide scanner and Imagescope software (Aperio, Vista, CA).
Slides were scanned at ×20 magnification using a ScanScopeXT slide scanner (Aperio Technologies, Vista, CA, USA).
Conventional immunohistochemical slides were digitalized for image analysis using a digital slide scanner (Aperio Technologies Inc., Vista, CA, USA).
For digital IHC quantification, slides were scanned at 40 × magnification (0.25 microns/pixel) using a whole slide scanner (ScanScope CS, Aperio Technologies, Vista, CA, USA) fitted with a 20x/0.75 Plan Apo objective lens (Olympus, Center Valley, PA, USA).
TMA slides were scanned at 40 × magnification (0.0625 μm/pixel) using a whole slide scanner (ScanScope CS or ScanScope XL; Aperio ePathology, Leica Biosystems, Vista, CA) and preprocessed using the Genie Histology Pattern Recognition software suite (Aperio) to segment tissues into three user-defined Image Classes (tumor, stroma, glass) as previously reported [ 28].
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