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After 72 h, the mixture was filtered using a sintered funnel and the extract (filtrate) concentrated using a rotary evaporator.
The content was then filtered using a sintered funnel, (which is equivalent to four folds of bandage or sheet of cheese cloth).
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The blue precipitate was filtered using a sintered glass funnel.
The synthesized peptides were cleaved from the resin by stirring for 2.5 h at 20°C in TFA (trifluoroacetic acid)/ethanedithiol/tri-isopropylsilane/water (97:1:1:1, by vol)., then separated from the resin by filtration under pressure using a glass-sintered funnel and precipitated with diethyl ether several times.
Upon completion, the reaction mixture was filtered through a sintered funnel, and the residue was washed with iPrOH/H2O (3:1), EtOH, and light petroleum ether and then dried under vacuum.
The reaction mixture was kept in an ultrasound bath in the dark for 30 min. Then, the resulting suspension was quickly filtered over a sintered funnel and the precipitate was washed with a minimum amount of DMF/pyridine solution.
The reaction mixture was kept in an ultrasound bath in the dark for 30 min. Then, the resulting suspension was quickly filtered over a sintered funnel and the precipitate was washed with minimum amount of DMF/pyridine solution.
Finally, oil and solvent were separated using a separatory funnel.
The extracts were filtered using a Büchner funnel and Whatman No. 1 filter paper.
The resulting lower layer was removed using a separating funnel and discarded.
Before chromatography analysis, the sample was extracted using a separating funnel.
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