The variant proteins were all purified as for the wild-type enzyme and were assayed using a single-turnover kinetic assay with radioactively labelled repeat RNA as a substrate.
Fm ST) is obtained using a rapid series of short flashlets to induce a single turnover of the PSII pool, whereas Fm MT) is measured during a much longer series of flashlets, during which multiple turnovers of the system completely reduce the plastoquinone (PQ) pool.
The Giants did not commit a single turnover in two games against the Eagles.
In 32 minutes at point guard, TERESA WEATHERSPOON had 10 assists, 7 rebounds and a single turnover.
Dick LeBeau's vaunted defense has yet to create a single turnover, but the Steelers, coming off a bye, have had two weeks to prepare for the turnover-prone Jets rookie quarterback Geno Smith and to stew over their many 2013 failures.
This rapid burst phase of the reaction measures a single turnover of the enzyme.
He's also a big part of why the team hasn't had a single turnover in its previous five games.
The time course of a single turnover for individual [PPi] was fitted to an exponential equation (Equation 1).
If radical pair recombination is required after each turnover, one might expect either deactivation after a single turnover or a substantial reduction in turnover of E287Q under aerobic conditions.
In the former instance, the cluster would be degraded concomitant with turnover, implying a single turnover reaction, whereas in the latter instance, multiple turnovers can ensue if the ligated sulfur source can be replenished.
