Sentence examples for using a single starting from inspiring English sources
We present a dual CRISPR tool, DECKO, which is cloned using a single starting oligonucleotide, thereby affording simplicity and scalability to CRISPR knockout studies of non-coding genomic elements, including long non-coding RNAs.
This system, DECKO (Double Excision CRISPR Knockout), is novel for the fact it expresses dual gRNAs from a single plasmid, which is cloned using a single starting oligonucleotide.
While the first condition alone has been met by a number of recent approaches [ 26– 28], the present study describes a method that fulfills both by cloning a dual gRNA expressing plasmid using a single starting oligonucleotide.
This pattern of hippocampal activation was also obtained in a second version of the task that only used a single start arm instead of multiple start arms.
The key advance presented here is the use of a single starting oligonucleotide for cloning a dual gRNA expression vector, conferring both convenience and scalability.
Activation and inactivation curves of the outward currents activated by depolarization were obtained using a single protocol: starting from a holding potential of −70 mV, a series of 1 s duration pre-pulses ranging from −90 to +50 mV were applied, followed by a 200 ms pulse test at +50 mV.
Set up a single starting location.
First, there is the issue of using a single seed to start the process, along with the selection of the seed research.
The judge then ordered state officials to instead start using a single large dose of barbiturate, common in animal euthanasia.
Then, in what legal experts said was a first, the judge instead ordered the state to start using a single large dose of barbiturate, common in animal euthanasia.
But in what legal experts said was a first, the judge ordered the state to start using a single large dose of barbiturate, the alternative method that opponents of the death penalty had unsuccessfully urged on the Supreme Court and that is common in animal euthanasia.
