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The parameters of the HMM can be initialised by using a single sequence or a pre-existing alignment.
Using a single sequence for each OTU, evolutionary distances were calculated with the Jukes-Cantor (tree-Markov) model and phlyogenetic trees determined by the Neighbour-Joining method, with 1000 trees generated.
Given heterozygous nucleotide sites in β-globin, data analyses were conducted on inferred haplotype states rather than using a single sequence with ambiguous nucleotide codes for heterozygous sites.
Primers were designed based on simultaneous viewing of the Se-Al file and an Oligo 4.02 (Rychlik, 2002) file, using a single sequence from the pool.
This suggests that using information from multiple MRI sequences to characterise BMLs may provide more prognostic information than using a single sequence alone.
We have shown that a traditional mapping approach using a single sequence as reference is incapable of dealing with the great allelic differences of HLA genes, with a large number of sequencing reads being missed.
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Using a single sequencing primer, a total of 3 CpG dinucleotides were sequenced for each Satellite 1 element targeted.
We report a complete genome sequence for C. stercorarium DSM8532T, generated using a single sequencing platform and in silico gap-filling.
However, as it is now possible to sequence multiple microbial genomes in a single day at low cost using a single sequencing machine [ 3], it is no longer feasible to manually curate the annotation of all sequenced genomes.
This protocol resulted in a high specificity for the targeted regions, with approximately 90% of the 454 reads mapping to the regions present on the enrichment array, allowing accurate detection of SNPs and point mutations in the genes of interest using a single sequencing run.
On the other hand, since the use of two positive RNAi systems suggests that Wang et al. were aware of the potential weaknesses of the system, this raises the question as to why they only used a single sequence for their RNAi control experiments.
More suggestions(15)
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using a single image
using a single antenna
using a single parameter
using a single silicon
using a single lens
using a single hydrophone
using a single light
using a single nozzle
using a single line
using a single sentence
using a single file
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