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We demonstrate the approach by introducing the fungal rubrofusarin polyketide pathway at a gene load of 11 kb distributed on three different plasmids, using a single selection trait and one yeast transformation step.
The polyA samples discussed thus far were prepared using a single selection with oligo-dT magnetic beads.
In fact, the largest enrichments were seen in studies using a single selection criterion.
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We initially used a single selection procedure to identify studies for inclusion in either of the two Cochrane Reviews in this series.
In contrast with other improvements on the SELEX method, which use a greater number of selection steps with the native protein [40], [41] or the recombinant protein [42], we used a single-selection step with a preset amplification of the library.
Note that these LR models were obtained from one data set using a single LogitBoost selection, and - given also the degree of correlation among variables - alternative models with equal performance may be selected by varying selection heuristics.
More advanced knockout constructs additionally carry loxP sites on both sides of the Bsr cassettes for subsequent removal of the selection marker by transient expression of Cre recombinase, thus allowing generation of multiple knockouts using just a single selection marker.
In plant breeding, there are two primary applications for DNA markers in selection: 1) selection of known genes using a single marker assay (marker-assisted selection; MAS); and 2) whole-genome profiling and prediction (genomic selection; GS).
This paper introduces RRegrs as a new computer-aided model selection framework using a single R function call.
Fifteen selection cycles were considered, using a single pair mating design.
Single-mode selection capability was achieved by using a comb configuration, and the triple transition interference (TTI) was suppressed by using a single phase unidirectional transducer (SPUDT).
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