Sentence examples for using a single primer from inspiring English sources

It is useful to simultaneously identify species in different genera using a single primer pair.

When large blind samples must be analyzed, it is useful to simultaneously identify species in different genera using a single primer pair.

A RAPD analysis performed using a single primer targeted to the pediocin AcH/PA-1 gene was carried out on several P. acidilactici strains and on some related species of lactic acid bacteria.

Such a palindromic sequence, if synthesized in the elongation assays, can be amplified using a single primer.

The Klenow reaction product is used as template for PCR using a single primer representing the 20 nt of the chimeric reverse transcription oligonucleotide.

Briefly, total genomic DNA was digested with a restriction enzyme (Nsp1), ligated to an appropriate adapter for the enzyme, and subjected to PCR amplification using a single primer.

Secondly, the amplification of a multi-gene family is necessarily problematic since not all loci and not all alleles at a locus will be detected using a single primers set.

To amplify the entire exon 2 of the DQB in the Japanese black bear, we initially used a single primer set URDQBex2-F1 and URDQBex2-R1 [ 39].

Because C1 and C2 are derived from the same exon, and because we used a single primer to clone both C1- and C2-containing variants, which did not contain an epitope tag, we are currently unable to detect FGFR2-C2 proteins.

The products were first amplified using a single upstream primer in combination with multiple downstream primers (see Materials and Methods).

Both methods greatly expand the technique suggested by Oetting et al. (1995), which only allowed up to five primer pairs to be multiplexed using a single universal primer.