Sentence examples for using a single pcr from inspiring English sources
Complete recoding of genes being costly, the reengineering their 5′-end using a single PCR megaprimer designed to comply with codon usage of the host was attempted.
All isolates were investigated for the presence of the lukS-PV and lukF-PV genes encoding PVL using a single PCR method as previously described [22].
Detection of T. gondii DNA was carried out using a single PCR assay targeting the GRA6 region.
Studies using hybridisation techniques or a combination of different PCR assays described a higher incidence than studies using a single PCR assay [ 1, 2, 14, 16].
Primers flanking the targeted exons (sequence on request) were used to amplify the cDNA as described previously for dystrophin, but using a single PCR for 35 cycles.
We applied a SDM method based on in vivo homologous recombination using a single PCR step, which is both simple and efficient [ 18, 25].
Third, we have expanded the repertoire of drug resistance cassettes available in pRS plasmids and, hence, the number of markers that can be amplified using a single pair of oligonucleotides for PCR-mediated gene replacement.
PCR amplification using a single pair of primers consistently recovered the 648 bp region near the 5′ terminus of the mitochondrial cytochrome c oxidase I (COI) gene that serves as the barcode region for the animal kingdom (Hebert et al. 2003).
specific primer pair [1] on material originating from ear-vein blood and stored on Whatman FTA filter cards [9]; b) ear-vein blood samples on Whatman FTA cards amplified using a single nested PCR targeting the Internal Transcribed Spacer (ITS) regions of ribosomal DNA [2].
In terms of methodology, our strategy allows small-pool PCR using a single-tube PCR setup, facilitated by time-release PCR (Slebos et al, 2004b), that is amendable to robotic automation.
Alternative splicing analysis of Col2a1 splice forms has often relied upon semi-quantitative PCR, using a single set of PCR primers to amplify multiple splice forms.
