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In this study, using a single gene manipulation approach that has allowed us, over the past decade, to recreate natural allelic diversity in the aliphatic GSL pathway, we have shown that GSL genetic variation at numerous loci directly impacts Arabidopsis fitness in the field (Table 1, Figure 7, Figure 7 figure supplement 1, Figure 8).
Jones's group has already engineered a blight-resistant potato, using a single gene it cloned from one found in a wild potato plant.
The present results proved that the novel DNA markers provided fast and accurate markers for different animal phylogenetic branches and that these convenient markers can also distinguish closely related species and hybrids using a single gene family tool.
Using a single gene reference resulted in an RV of 1.48.
Shared genes in different strains were calculated using the Phylogenetic profile program using a single gene profile from the Integrated Microbial Genome server.
Therefore, contigs were extended using GenomeWalker (Clontech, Mountain View, CA) ligation mediated PCR using a single gene specific primer for each contig end.
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"This is the first time anyone's used a single gene to implant something as complex as a behavioral trait into another species," says Young. "It's a brilliant piece of work," says Dean Hamer, a behavioral geneticist at the National Cancer Institute.
Conventional methods that use gene expression to quantify biological processes must use a single gene or a set of well-correlated genes, but solving a system of gene expression equations surmounts this limitation.
Smaller values of q produced more accurate results for modules that use multiple genes in multiple tissues, while higher values of q produced more accurate results for modules that use a single gene in many tissues.
To avoid inconsistencies and to get uniformity in the resultant data, we used a single gene finding program.
Therefore, the two variables will be the choice of alignment – whether to use a single gene [ 26, 27] or concatenate several genes together [ 6- 8] – and the choice of model.
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