Sentence examples for using a single dna from inspiring English sources

Figg points out that his laboratory recently completed a study using a DNA chip that can rapidly profile 1243 single-nucleotide polymorphisms in 163 genes involved in drug metabolism and transport using a single DNA sample.

It is therefore possible to disrupt both genes using a single DNA construct, as has been shown for other paralogous genes in rodent malaria [18], [52] and for adjacent genes encoding aspartatic proteases in P. falciparum [53], [54].

Probing for U6 was performed using a mix of DNA oligonucleotides complementary to U6, whereas probing for let-7 was performed using a single DNA oligonucleotide complementary to the mature let-7 sequence.

Instead, of using a single DNA alphabet at the randomly chosen index i, we select the a subsequence around the chosen indices.

The simplest method of making a transgenic animal is to use a single DNA construct in which a promoter directly regulates the expression of a transgene.

Canonical base pairing has been used to create duplex DNA branches on the ends of frayed wires[49], but initial assembly of the frayed wires exploits only Hoogsteen hydrogen bonding and used a single DNA sequence, which does not allow significant variability/flexibility[49].

Moreover, using a single type of DNA marker to construct a lotus linkage map would easily lead to uneven marker distribution and large intervals between adjacent markers.

Genomic DNA was prepared from 141 isochromosomal lines of Drosophila pseudoobscura using a single-fly DNA extraction protocol (Gloor and Engels 1992).

It was enzymatically generated using a single-stranded DNA library synthesized in-house as template for a one-cycle fill-in reaction with Vent exo− DNA polymerase (New England Biolabs) and the T7 RNA polymerase promotor-containing forward primer 5′-TCTAATACGACT-CACTATAGGAGCTTAGACAACAGCAG-3′, followed by in vitro transcription with T7 RNA polymerase (Invitrogen).

Unlike PCR which requires thermal cycles, RCA is an isothermal probe amplification strategy using a single-stranded DNA as a padlock probe (Fig. 2A).

Our approach used only a single DNA sample from a single individual to represent each inbred strain.