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In addition the performance of the guide was compared to the standard slit setup at STRESS-SPEC using a single bead weld sample used in earlier round robin tests for residual strain measurements.
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The tumor samples were homogenized in RLT-ME buffer (Qiagen, Germantown, MD) using a single 5 mm steel bead per sample in a Tissuelyser (Qiagen, Germantown, MD) for two minutes at 28 Hz.
One limitation of our technique stems from using a single image to determine which beads are within the image plane.
The polyA samples discussed thus far were prepared using a single selection with oligo-dT magnetic beads.
Single-stranded PCR products (see Fig. 4 for details) were obtained by using a single biotinylated primer, immobilization on streptavidin beads, and denaturation with NaOH.
The resulting library was subjected to emPCR and used to prepare DNA beads for sequencing using a single region of a four region picotiter plate on a Roche GS-FLX instrument using Titanium chemistry (454 Life Sciences).
The silicon membrane is held in place using a single static trap directed at the attached functionalized bead.
We purified Drep-2GFP from fly heads using a single chain anti-GFP antibody coupled to agarose beads.
Using a single booster.
Excitation point spread functions (PSFs) were measured using stage-scanning confocal imaging of a single subdiffraction sized fluorescent bead using a single-pixel hybrid photodetector (HPD) (Model R7110U-40UF, Hamamatsu Photonics, Bridgewater, NJ) as described in [ 21] for single spot excitation.
If you don't use a charm, place a single bead in the center of the ribbon (threading it over only one tail), and then fold the ribbon in half and insert both tails through the remaining beads to make it secure.
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