Sentence examples for using a single amplification from inspiring English sources

Microsatellite mutations were detected similarly to the method described by Coolbaugh-Murphy et al (Coolbaugh-Murphy et al, 2004), with the main difference of using a single amplification reaction using time-release PCR (Slebos et al, 2004b).

The complete genome was amplified using a single amplification method with primers P1 (1821–1841 from EcoRI site) and P2 (1825–1806 from EcoRI site), with modifications in the cycling conditions [ 30].

From 1,505 env sequences obtained using a single genome amplification approach we show that 78% of infections involved single variant transmission and 22% involved multiple variant transmissions (median of 3).

The mean expression level of the enhanced green fluorescent protein (eGFP) used herein as a model protein, increased up to eight-fold using a single round of amplification in the case of adherent colonies formation and up to 4.5-fold in the case of suspension polyclonal cultures.

200 ng of total RNA were used for a single amplification.

Briefly, total genomic DNA was digested with a restriction enzyme (Nsp1), ligated to an appropriate adapter for the enzyme, and subjected to PCR amplification using a single primer.

The resulting first strand cDNA products were poly G-tailed followed by 30 rounds of PCR amplification using a single primer.

Briefly, kDNA amplicons were subjected to electrophoresis in 1.5% agarose gel (1.0% agarose, 0.5% agarose low melting point), punctured from the gel, diluted 10 times in double distilled water, and submitted to a second step of low stringency amplification, using a single primer (S35G: 5'-ATGTACGGGGAGATGCATGA-3').

The same person did the RAPD screening and amplification using a single protocol.

The PCR reactions were carried out the same as for the FISH probe amplification, using a single primer or combinations of two primers (14F/34R, 34F/14R, 34R/Tel, 14R/Tel).

WGSA is used to reduce the complexity of the sample, and involves ligating a linker to restriction-digested DNA, which enables PCR amplification using a single primer that is complementary to the adaptor.