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Data generated by NGS were exploited using a simple BLAST approach.
Page 8:"Using a simple BLAST threshold criterion, a total of 418,214 homologous sets of proteins was identified; over half of these (255,417) were orphan proteins".
For example, we first identified 85 tandem clusters in C. albicans SC5314 using a simple BLAST approach, and this was increased to 106 using synteny information, whereas only 24 were reported in Butler et al [ 5].
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We also provide a pipeline for validation of the array in other species using a simple BLAST-based sequence identify filter.
Using a simple PSI-BLAST search cannot detect N. crassa GH43-2 without significant phylogenetic support.
A consensus sequence for a subgroup of Tc1 elements of our interest (1,610 nt, supplementary fig. S1, Supplementary Material online) was generated from the top 300 blast hits using a simple majority rule as practiced previously (Pace et al. 2008) and was subsequently used as a query to search for closely related sequences in the aforementioned sequence data using BLASTN.
By using a simple uni-axial numerical model, this study shows that spalling instead might occurs when the cyclic response from a blast wave gradually increase the inelastic strains in the concrete.
It uses BLAST scores to assign statistical significance, matches pairs that reach that level of significance, then constructs clusters using a simple, greedy, single-linkage clustering method.
SNPs were called using a simple thresholding.
Without using a weighting scheme, the LZW-BLAST method uses a simple multiplication rule to combine the LZW and BLAST scores (Kocsor et al., 2006).
The missile's warhead is made with a mixture of chemicals rather than a simple blast mechanism.
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