The Serengeti-transformed DNA sample was sequenced at ILRI using a similar sequencing strategy, except that the mate-paired library was prepared with 3 Kb inserts.
A recent study using a similar sequencing approach applied to A. gambiae mosquitoes pre- and post-bloodmeal revealed 58 novel miRNAs, showing the utility of a targeted deep sequencing approach [ 43]. 18 of these miRNAs were also revealed by our analysis, ultimately reducing the number of truly novel miRNAs in our study to 45 (Additional file 5).
Token-based techniques (Li et al. [2006]; Leitao [2004]; Basit et al. [2007]) use a similar sequence matching algorithm.
Shen et al. also used a similar sequence involving temporary protection of compound 405 with levulinates to prepare the triciribine prodrug 407 (Scheme 117).
Although Sciarra et al. reported a Se of 71 84% and a Spe of 83 88% in the detection of local recurrence after RP using a similar MRSI sequence, the results of Roy's study are disappointing most likely because Cit could not be measured since after removal of prostatic gland, a metabolite ratio is difficult to calculate.
Furthermore, Maricic et al. 2010 [ 41] reported up to a four-fold difference in the number of sequence reads obtained using captured mitochondrial DNA sequences from 46 human individuals using a similar bait-design sequence capture system.
Our findings are consistent with a recent report using a similar next-generation sequencing platform as ours to compare RNA from paired FF and FFPE tissue from a variety of tumor types to report a correlation coefficient of 0.90 [ 14].
To replicate data from the Papworth cystic fibrosis outbreak clusters 1 and 2 (6 ) by using a similar approach, we mapped sequencing reads from the subset of 6 Papworth isolates, together with reads with from the 3 Seattle cystic fibrosis isolates and soft tissue strain CRM-0020 from Brazil (Table 1), onto the M. abscessus type strain ATCC 19977T reference genome by using BWA software (25 ).
Other studies in barley [ 7], salmon [ 8], and melon [ 9] have been carried out using a similar BAC pooling and 454 sequencing strategy that allows for high quality sequencing of sub-genomic regions of high priority (e.g. QTL intervals or poorly resolved WGS assembly regions) at a cost far less than that of whole-genome sequencing.
Using a similar strategy, all 5D gene sequence reads conserved with all three model grasses but lying outside the 5D syntenic regions described above were analysed further for evidence of possible wheat-specific rearrangements.
