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In addition, we repeated the comparison of Wilms patients before and after therapy using all 43 samples and applying unpaired two-tailed t-test, which confirmed the results of the matched samples (no significant miRNA using a significance threshold of 0.05 and FDR adjustment).
Using a significance threshold level, Bonferroni corrected to, we get in Table 4 significant differences between the sample groups.
Overall we observed no significant associations between SNPs in telomere maintenance genes and mortality using a significance threshold of p=0.001.
DAVID EASE was also used to analyze the gene sets for each major temporal group (increased, variable, decreased expression) to find significant known pathways determined by KEGG[28] and GenMAPP[29] databases, using a significance threshold of p<0.05.
The final basic regression models were decided with a backwards elimination procedure using a significance threshold of ≥0.1 for elimination of excessive independent variables.
The normalization factor is the magnitude of the correct flow, it is taken into account the effect of small flows using a significance threshold T as shown in Equation (16).
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We used a significance threshold of p ≤ 0.05 to select significant mapping associations.
All analyses used a significance threshold of α < 0.05 (two-tailed).
Stepwise model building procedures used a significance threshold of 0.05 for both entry and retention in the models.
In an attempt to maximise our chances of detecting illness-relevant changes in methylation, we used a significance threshold of FDR-adjusted p ≤ 0.1.
We used a significance threshold (−log10 P-value)) determined by 2000 permutation tests to reach a global type I risk of 10% genome-wide.
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